近日,司法鉴定科学研究院严慧课题组汤以绫老师,使用IPHASE品牌产品:II相代谢稳定性试剂盒(UGTs)在《Journal of Pharmaceutical and Biomedical Analysis》权威期刊上发表文章《Metabolism of dipentylone in zebrafish and human liver microsomes determined by liquid chromatography–high resolution mass spectrometry 》,影响因子3.4!
本论文中,利用LC-HRMS分析dipentylone在斑马鱼和HLM体内和体外的代谢,并成功鉴定了代谢产物及其主要代谢途径,选择了合适的代谢物作为生物标志物。
摘要
The consumption of novel psychoactive substances (NPS) is exceedingly prevalent in society, as these substances are sold and distributed as “legal highs.” One novel synthetic cathinone emerging in the market is 1-(1,3-ben-zodioxol-5-yl)–2-(dimethylamino) pentan-1-one (dipentylone). The goal of this work was to study the in vivo and in vitro metabolism of dipentylone in zebrafish and human liver microsomes (HLMs) by liquid chromatography-high resolution mass spectrometry (LCHRMS). The zebrafish and HLM samples contained 14 dipentylone metabolites, specifically 12 phase I metabolites and 2 phase II metabolites. The main metabolic pathways included monohydroxylation (M1 and M2), N-dealkylation (M3), hydroxylation of the aromatic ring and dealkoxylation of M3 (M4), O-dealkylation (M5), N-dealkylation of M5 (M6), reduction of carboxide (M7), monohydroxylation of M5 (M8), dehydrogenation (M9), dealkoxylation (M10), N-dealkylation of M10 (M11), dealkoxylation of M9 (M12), glucuronidation of M5 (M13), and sulfation (M14). The monohydroxylated metabolite (M2) can be recommended as metabolic markers for dipentylone. This study is the first to identify a target compound for monitoring the abuse of dipentylone and to determine the essential chemical structure of the metabolites for urther toxicological research.
完整版文献可在【IPHASE】公众号后台留言【获取文章】
再次恭喜文献发表,以及对我司产品的认可,希望以上文献能帮助大家了解目前研究进展及我们的核心技术,欢迎各位新老客户联系我们咨询、提出意见,愿我们的努力成果与您的科研碰撞出不一样的火花!
发 文 章 得 奖 励
凡使用本公司产品,在国内及国际刊物上发表论文(论文发表日起一年内),并注明产品属于IPHASE BIOSCIENCES CO,LTD / 汇智和源生物技术(苏州)有限公司所有,即可申请奖励。根据发表刊物影响因子不同,给予不同金额奖品:
非SCI论文及IF≤5分,500元礼品;
5分<IF≤8分 800元;
8分<IF≤10分 1000元;
IF≥10分 2000元;
注:礼品卡也可兑换同等金额产品购买抵用券;
活动多多,礼品丰厚,快来参与吧!
关 于 我 们
汇智和源,致力于为创新药研发企业及生命科学研究机构提供高品质的生物试剂,IPHASE为公司核心品牌,品牌宗旨“Innovative Reagents For Innovative Research”。